Reduced sulfhydryls maintain specific incision of BPDE-DNA adducts by recombinant thermoresistant Bacillus caldotenax UvrABC endonuclease. [electronic resource]
- Protein expression and purification Sep 2003
- 88-98 p. digital
Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
1046-5928
10.1016/s1046-5928(03)00137-2 doi
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide--analysis Bacillus--enzymology Chitin--chemistry Chromatography, Liquid Cloning, Molecular DNA Adducts--analysis DNA Damage DNA Helicases--biosynthesis DNA Repair DNA, Superhelical--chemistry Deoxyribonucleases--metabolism Dithiothreitol--chemistry Electrophoresis, Agar Gel Electrophoresis, Polyacrylamide Gel Endodeoxyribonucleases--biosynthesis Enzyme Stability Escherichia coli--genetics Escherichia coli Proteins--biosynthesis Gene Expression Regulation, Bacterial--drug effects Genetic Vectors--genetics Hot Temperature Isopropyl Thiogalactoside--pharmacology Nucleic Acid Conformation--drug effects Plasmids--analysis Recombinant Proteins--chemistry Spectrometry, Fluorescence Substrate Specificity Sulfhydryl Compounds--chemistry Time Factors