TY - GEN AU - Siggelkow,Heide AU - Schenck,Michael AU - Rohde,Manfred AU - Viereck,Volker AU - Tauber,Simone AU - Atkinson,Michael J AU - Hüfner,Michael TI - Prolonged culture of HOS 58 human osteosarcoma cells with 1,25-(OH)2-D3, TGF-beta, and dexamethasone reveals physiological regulation of alkaline phosphatase, dissociated osteocalcin gene expression, and protein synthesis and lack of mineralization SN - 0730-2312 PY - 2002///0722 KW - Adult KW - Alkaline Phosphatase KW - genetics KW - Animals KW - Blotting, Northern KW - Calcification, Physiologic KW - Calcitriol KW - pharmacology KW - Collagen Type I KW - Core Binding Factor alpha Subunits KW - DNA Primers KW - chemistry KW - DNA-Binding Proteins KW - metabolism KW - Dexamethasone KW - Gene Expression KW - Gene Expression Regulation, Enzymologic KW - Humans KW - Male KW - Mice KW - Microscopy, Electron, Scanning KW - Osteocalcin KW - Osteosarcoma KW - drug therapy KW - RNA, Messenger KW - Rats KW - Reverse Transcriptase Polymerase Chain Reaction KW - Transcription Factors KW - Transforming Growth Factor beta KW - Tumor Cells, Cultured KW - drug effects N1 - Publication Type: Comparative Study; Journal Article UR - https://doi.org/10.1002/jcb.10122 ER -