Self-cloning minitransposon phoA gene-fusion system promotes the rapid genetic analysis of secreted proteins in gram-negative bacteria. [electronic resource]
- BioTechniques Sep 2000
- 470-2, 474 p. digital
Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
0736-6205
10.2144/00293bm12 doi
Alkaline Phosphatase--genetics Bacterial Proteins--genetics Cloning, Molecular Cyclin-Dependent Kinases--genetics DNA Restriction Enzymes--metabolism Deoxyribonucleases, Type II Site-Specific Escherichia coli--genetics Escherichia coli Proteins Gram-Negative Bacteria--genetics Mutagenesis Polymerase Chain Reaction Recombinant Fusion Proteins