An approach to optimizing the active site in a glutathione transferase by evolution in vitro. [electronic resource]
- The Biochemical journal Nov 1999
- 93-100 p. digital
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
0264-6021
Amino Acid Substitution Base Sequence Catalytic Domain--genetics DNA Primers--genetics Dinitrochlorobenzene--metabolism Directed Molecular Evolution Genetic Variation Glutathione Transferase--chemistry Humans In Vitro Techniques Kinetics Models, Molecular Mutagenesis, Site-Directed Protein Structure, Secondary Substrate Specificity