TY - GEN AU - Chou,C P AU - Yu,C C AU - Tseng,J H AU - Lin,M I AU - Lin,H K TI - Genetic manipulation to identify limiting steps and develop strategies for high-level expression of penicillin acylase in Escherichia coli SN - 0006-3592 PY - 1999///0426 KW - Cloning, Molecular KW - methods KW - DNA, Ribosomal KW - Escherichia coli KW - enzymology KW - Gene Expression Regulation, Bacterial KW - Gene Expression Regulation, Enzymologic KW - Genes, Bacterial KW - Kinetics KW - Penicillin Amidase KW - biosynthesis KW - Plasmids KW - Protein Biosynthesis KW - Regulatory Sequences, Nucleic Acid KW - Restriction Mapping KW - Serratia marcescens KW - Transcription, Genetic N1 - Publication Type: Journal Article; Research Support, Non-U.S. Gov't ER -