Phospholipase A2 engineering. Deletion of the C-terminus segment changes substrate specificity and uncouples calcium and substrate binding at the zwitterionic interface. [electronic resource]
Producer: 19961029Description: 12164-74 p. digitalISSN:- 0006-2960
- Amino Acid Sequence
- Animals
- Binding Sites
- Calcium -- metabolism
- Calorimetry
- Cattle
- Cloning, Molecular
- Computer Graphics
- DNA Primers
- Enzyme Stability
- Escherichia coli
- Magnetic Resonance Spectroscopy
- Models, Molecular
- Mutagenesis, Site-Directed
- Pancreas -- enzymology
- Phospholipases A -- biosynthesis
- Phospholipases A2
- Point Mutation
- Protein Conformation
- Protein Denaturation
- Protein Engineering
- Recombinant Proteins -- biosynthesis
- Sensitivity and Specificity
- Sequence Deletion
- Substrate Specificity
- Thermodynamics
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Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
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