Recombinant canditropsin, an extracellular aspartic protease from yeast Candida tropicalis. Escherichia coli expression, purification, zymogen activation, and enzymic properties. [electronic resource]
Producer: 19931020Description: 20143-7 p. digitalISSN:- 0021-9258
- Amino Acid Sequence
- Aspartic Acid Endopeptidases -- biosynthesis
- Base Sequence
- Candida -- enzymology
- Chromatography, High Pressure Liquid
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Enzyme Activation
- Escherichia coli -- genetics
- Gene Expression
- Genetic Vectors
- Hydrogen-Ion Concentration
- Insulin -- metabolism
- Kinetics
- Molecular Sequence Data
- Molecular Weight
- Oligodeoxyribonucleotides
- Peptide Fragments -- isolation & purification
- Polymerase Chain Reaction
- Protease Inhibitors -- pharmacology
- Recombinant Proteins -- biosynthesis
- Substrate Specificity
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Publication Type: Journal Article; Research Support, U.S. Gov't, P.H.S.
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