Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes. [electronic resource]
Producer: 20180322Description: 16-28 p. digitalISSN:- 1095-9130
- Animals
- Bacterial Proteins -- genetics
- Base Sequence
- CRISPR-Associated Protein 9
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- DNA -- genetics
- Electroporation
- Endonucleases -- genetics
- Gene Editing -- methods
- Gene Targeting -- methods
- Gene Transfer Techniques
- Genome
- HEK293 Cells
- Humans
- Jurkat Cells
- Lipids -- chemistry
- Mice
- Microinjections
- RNA, Guide, CRISPR-Cas Systems -- chemical synthesis
- Recombinant Proteins -- genetics
- Recombinational DNA Repair
- Ribonucleoproteins -- genetics
- Zygote -- cytology
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Publication Type: Journal Article
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