Evaluation and rational design of guide RNAs for efficient CRISPR/Cas9-mediated mutagenesis in Ciona. [electronic resource]
Producer: 20170911Description: 8-20 p. digitalISSN:- 1095-564X
- Animals
- Base Sequence
- CRISPR-Cas Systems
- Ciona intestinalis -- embryology
- Embryo, Nonmammalian -- embryology
- Gene Editing -- methods
- Gene Expression Regulation, Developmental
- Gene Targeting -- methods
- High-Throughput Nucleotide Sequencing -- methods
- Mutagenesis
- Mutation
- RNA, Guide, CRISPR-Cas Systems -- genetics
- Reproducibility of Results
- Sequence Homology, Nucleic Acid
- Transcriptome -- genetics
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Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural
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