Cloning, expression, purification and characterization of a single chain variable fragment specific to tumor necrosis factor alpha in Escherichia coli. [electronic resource]
Producer: 20120605Description: 238-44 p. digitalISSN:- 1873-4863
- Antibodies, Monoclonal -- genetics
- Apoptosis -- drug effects
- Cell Line, Tumor
- Chromatography, Affinity -- methods
- Cloning, Molecular -- methods
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli -- genetics
- Humans
- Recombinant Proteins -- biosynthesis
- Single-Chain Antibodies -- biosynthesis
- Sodium Chloride
- Substrate Specificity
- Tumor Necrosis Factor-alpha -- metabolism
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Publication Type: Journal Article; Research Support, Non-U.S. Gov't
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