Development and implementation of a miniaturized high-throughput time-resolved fluorescence energy transfer assay to identify small molecule inhibitors of polo-like kinase 1. [electronic resource]
Producer: 20080225Description: 723-35 p. digitalISSN:- 1540-658X
- Blotting, Western
- Cell Cycle Proteins -- antagonists & inhibitors
- Data Interpretation, Statistical
- Drug Design
- Drug Evaluation, Preclinical
- Enzyme Inhibitors -- chemical synthesis
- Fluorescence Resonance Energy Transfer
- Fluorometry
- Freezing
- Hydrogen-Ion Concentration
- Indicators and Reagents
- Kinetics
- Miniaturization
- Protein Kinase C -- antagonists & inhibitors
- Protein Serine-Threonine Kinases -- antagonists & inhibitors
- Proto-Oncogene Proteins -- antagonists & inhibitors
- Reproducibility of Results
- Silver Staining
- Substrate Specificity
- Temperature
- Polo-Like Kinase 1
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Publication Type: Journal Article; Research Support, N.I.H., Extramural
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