Isolation of mutant cells lacking Insig-1 through selection with SR-12813, an agent that stimulates degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase. [electronic resource]
Producer: 20041124Description: 43136-47 p. digitalISSN:- 0021-9258
- Animals
- CCAAT-Enhancer-Binding Proteins
- CHO Cells -- metabolism
- Cell Line
- Cell Nucleus -- metabolism
- Cholesterol -- metabolism
- Cricetinae
- DNA -- chemistry
- DNA-Binding Proteins
- Diphosphonates -- pharmacology
- Dose-Response Relationship, Drug
- Endoplasmic Reticulum -- metabolism
- Fatty Acids -- metabolism
- Fibroblasts -- metabolism
- Humans
- Hydroxymethylglutaryl CoA Reductases -- metabolism
- Immunoblotting
- Intracellular Signaling Peptides and Proteins
- Lipid Metabolism
- Membrane Proteins -- genetics
- Models, Biological
- Mutagenesis, Site-Directed
- Mutation
- Peptides -- chemistry
- Plasmids -- metabolism
- RNA Interference
- Reverse Transcriptase Polymerase Chain Reaction
- Sterol Regulatory Element Binding Protein 1
- Sterols -- metabolism
- Subcellular Fractions
- Temperature
- Time Factors
- Transcription Factors
- Transfection
- Ubiquitin -- metabolism
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Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
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