Development of a fed-batch fermentation process to overproduce phosphoenolpyruvate carboxykinase using an expression vector with promoter and plasmid copy number controllable by heat. [electronic resource]
Producer: 20040720Description: 459-66 p. digitalISSN:- 0006-3592
- Bacteriophage T7 -- genetics
- Bioreactors -- microbiology
- Cell Culture Techniques -- methods
- Cell Division -- radiation effects
- Escherichia coli -- cytology
- Feasibility Studies
- Fermentation -- physiology
- Gene Dosage
- Gene Expression Regulation, Bacterial -- radiation effects
- Gene Expression Regulation, Enzymologic -- radiation effects
- Heat-Shock Response -- physiology
- Hot Temperature
- Isopropyl Thiogalactoside -- genetics
- Phosphoenolpyruvate Carboxykinase (ATP) -- biosynthesis
- Plasmids -- genetics
- Promoter Regions, Genetic
- Protein Engineering -- methods
- Recombinant Fusion Proteins -- biosynthesis
- Transfection -- methods
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Publication Type: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
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