Genetic manipulation to identify limiting steps and develop strategies for high-level expression of penicillin acylase in Escherichia coli. [electronic resource]
Producer: 19990426Description: 263-72 p. digitalISSN:- 0006-3592
- Cloning, Molecular -- methods
- DNA, Ribosomal
- Escherichia coli -- enzymology
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Genes, Bacterial
- Kinetics
- Penicillin Amidase -- biosynthesis
- Plasmids
- Protein Biosynthesis
- Regulatory Sequences, Nucleic Acid
- Restriction Mapping
- Serratia marcescens -- enzymology
- Transcription, Genetic
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Publication Type: Journal Article; Research Support, Non-U.S. Gov't
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