Site-directed mutagenesis of a disulfide bridge in cathepsin D: expression, activation, purification, and characterization.

Beyer, B M

Site-directed mutagenesis of a disulfide bridge in cathepsin D: expression, activation, purification, and characterization. [electronic resource] - Advances in experimental medicine and biology 1995 - 285-7 p. digital

Publication Type: Comparative Study; Journal Article

0065-2598

10.1007/978-1-4615-1871-6_33 doi


Amino Acid Sequence
Animals
Cathepsin D--biosynthesis
Cloning, Molecular
Cysteine
Disulfides
Enzyme Activation
Escherichia coli
Gene Expression
Molecular Sequence Data
Mutagenesis, Site-Directed
Oligopeptides--metabolism
Point Mutation
Polymerase Chain Reaction
Recombinant Proteins--biosynthesis
Substrate Specificity