Bacterial expression and purification of human protein prenyltransferases using epitope-tagged, translationally coupled systems.

Omer, C A

Bacterial expression and purification of human protein prenyltransferases using epitope-tagged, translationally coupled systems. [electronic resource] - Methods in enzymology 1995 - 3-12 p. digital

Publication Type: Journal Article

0076-6879

10.1016/0076-6879(95)50057-x doi


Alkyl and Aryl Transferases
Amino Acid Sequence
Base Sequence
Chromatography, Ion Exchange--methods
Cloning, Molecular--methods
DNA Primers
Epitopes
Escherichia coli
Humans
Indicators and Reagents
Kinetics
Macromolecular Substances
Molecular Sequence Data
Mutagenesis, Site-Directed
Plasmids
Polymerase Chain Reaction--methods
Protein Biosynthesis
Recombinant Proteins--biosynthesis
Transferases--biosynthesis