A new protocol to digest human IgM with papain that results in homogeneous Fab preparations that can be routinely crystallized.

Newkirk, M M

A new protocol to digest human IgM with papain that results in homogeneous Fab preparations that can be routinely crystallized. [electronic resource] - Hybridoma Oct 1987 - 453-60 p. digital

Publication Type: Journal Article; Research Support, U.S. Gov't, P.H.S.

0272-457X

10.1089/hyb.1987.6.453 doi


Amino Acid Sequence
Antibodies, Monoclonal--metabolism
Chromatography, Gel
Chromatography, Ion Exchange
Crystallization
Cysteine--metabolism
Humans
Hydrolysis
Immunoglobulin Fab Fragments--isolation & purification
Immunoglobulin M--analysis
Molecular Sequence Data
Papain--metabolism
Rheumatoid Factor--metabolism