Genome-Wide CRISPRi/a Screening in an In Vitro Coculture Assay of Human Immune Cells with Tumor Cells.

Zhang, Jialing

Genome-Wide CRISPRi/a Screening in an In Vitro Coculture Assay of Human Immune Cells with Tumor Cells. [electronic resource] - Methods in molecular biology (Clifton, N.J.) 2020 - 231-252 p. digital

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

1940-6029

10.1007/978-1-0716-0203-4_15 doi


CRISPR-Cas Systems--genetics
Coculture Techniques--methods
Genetic Testing
Genome
HEK293 Cells
High-Throughput Nucleotide Sequencing
Humans
Lentivirus--metabolism
Leukocytes--immunology
Neoplasms--pathology
Oligonucleotides--metabolism
Plasmids--metabolism
RNA, Guide, CRISPR-Cas Systems--metabolism
Transformation, Genetic
Virion--metabolism