Identifying Cellular Nonsense-Mediated mRNA Decay (NMD) Targets: Immunoprecipitation of Phosphorylated UPF1 Followed by RNA Sequencing (p-UPF1 RIP-Seq).

Kurosaki, Tatsuaki

Identifying Cellular Nonsense-Mediated mRNA Decay (NMD) Targets: Immunoprecipitation of Phosphorylated UPF1 Followed by RNA Sequencing (p-UPF1 RIP-Seq). [electronic resource] - Methods in molecular biology (Clifton, N.J.) 2018 - 175-186 p. digital

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

1940-6029

10.1007/978-1-4939-7540-2_13 doi


HEK293 Cells
High-Throughput Nucleotide Sequencing--methods
Humans
Immunoprecipitation--methods
Nonsense Mediated mRNA Decay--physiology
Phosphorylation--immunology
RNA Helicases--immunology
RNA, Messenger--genetics
Sequence Analysis, RNA--methods
Trans-Activators--immunology
Transcriptome--physiology