Efficient generation of hiPSC neural lineage specific knockin reporters using the CRISPR/Cas9 and Cas9 double nickase system.
Li, Shenglan
Efficient generation of hiPSC neural lineage specific knockin reporters using the CRISPR/Cas9 and Cas9 double nickase system. [electronic resource] - Journal of visualized experiments : JoVE May 2015 - e52539 p. digital
Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Video-Audio Media
1940-087X
10.3791/52539 doi
Cell Lineage
Clustered Regularly Interspaced Short Palindromic Repeats
Deoxyribonuclease I--chemistry
Gene Knock-In Techniques--methods
Gene Targeting--methods
Genetic Vectors
Homologous Recombination
Humans
Induced Pluripotent Stem Cells--cytology
Neural Stem Cells--cytology
Efficient generation of hiPSC neural lineage specific knockin reporters using the CRISPR/Cas9 and Cas9 double nickase system. [electronic resource] - Journal of visualized experiments : JoVE May 2015 - e52539 p. digital
Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Video-Audio Media
1940-087X
10.3791/52539 doi
Cell Lineage
Clustered Regularly Interspaced Short Palindromic Repeats
Deoxyribonuclease I--chemistry
Gene Knock-In Techniques--methods
Gene Targeting--methods
Genetic Vectors
Homologous Recombination
Humans
Induced Pluripotent Stem Cells--cytology
Neural Stem Cells--cytology