Separation and identification of methotrexate and its metabolites, 7-hydroxymethotrexate and polyglutamates, in human tissues by reversed-phase high-performance liquid chromatography coupled with radioimmunoassay.

Anzai, T

Separation and identification of methotrexate and its metabolites, 7-hydroxymethotrexate and polyglutamates, in human tissues by reversed-phase high-performance liquid chromatography coupled with radioimmunoassay. [electronic resource] - Journal of chromatography Apr 1987 - 445-9 p. digital

Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.

10.1016/s0378-4347(00)83241-7 doi


Brain Chemistry
Chromatography, High Pressure Liquid
Humans
Liver--analysis
Methotrexate--analogs & derivatives
Peptides--isolation & purification
Polyglutamic Acid--isolation & purification
Radioimmunoassay
Spectrophotometry, Ultraviolet