Development of a SYBR Green I based duplex real-time PCR for detection of bovine herpesvirus-1 in semen.

Pawar, Sachin S

Development of a SYBR Green I based duplex real-time PCR for detection of bovine herpesvirus-1 in semen. [electronic resource] - Journal of virological methods Nov 2014 - 6-10 p. digital

Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Study

1879-0984

10.1016/j.jviromet.2014.07.027 doi


Animals
Benzothiazoles
Cattle
Cattle Diseases--diagnosis
DNA Primers--genetics
Diamines
Growth Hormone--genetics
Herpesviridae Infections--diagnosis
Herpesvirus 1, Bovine--genetics
Multiplex Polymerase Chain Reaction--methods
Organic Chemicals--metabolism
Quinolines
Real-Time Polymerase Chain Reaction--methods
Semen--virology
Sensitivity and Specificity
Staining and Labeling--methods
Transition Temperature
Viral Proteins--genetics