Purification of an amide hydrolase DamH from Delftia sp. T3-6 and its gene cloning, expression, and biochemical characterization.

Wang, Fei

Purification of an amide hydrolase DamH from Delftia sp. T3-6 and its gene cloning, expression, and biochemical characterization. [electronic resource] - Applied microbiology and biotechnology Sep 2014 - 7491-9 p. digital

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

1432-0614

10.1007/s00253-014-5710-y doi


Amidohydrolases--chemistry
Cations, Divalent--metabolism
Chemical Precipitation
Chromatography, Gel
Chromatography, Ion Exchange
Cloning, Molecular
DNA, Bacterial--chemistry
Delftia--enzymology
Enzyme Activators--metabolism
Enzyme Inhibitors--metabolism
Enzyme Stability
Escherichia coli--enzymology
Gene Expression
Hydrogen-Ion Concentration
Kinetics
Mass Spectrometry
Molecular Sequence Data
Molecular Weight
Recombinant Proteins--chemistry
Sequence Analysis, DNA
Sequence Analysis, Protein
Substrate Specificity
Temperature