Production of functional soluble Dectin-1 glycoprotein using an IRES-linked destabilized-dihydrofolate reductase expression vector.

Ng, Say Kong

Production of functional soluble Dectin-1 glycoprotein using an IRES-linked destabilized-dihydrofolate reductase expression vector. [electronic resource] - PloS one 2012 - e52785 p. digital

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

1932-6203

10.1371/journal.pone.0052785 doi


Amino Acid Sequence
Animals
Antifungal Agents--chemistry
Bioreactors
CHO Cells
Cloning, Molecular
Cricetinae
Genetic Vectors
Glycoproteins--biosynthesis
Lectins, C-Type--biosynthesis
Methotrexate--pharmacology
Mice
Molecular Sequence Data
Peptide Chain Initiation, Translational
Peptide Fragments--biosynthesis
Plasmids--genetics
Protein Binding
Protein Engineering
Recombinant Fusion Proteins--biosynthesis
Saccharomyces cerevisiae--metabolism
Solubility
Tetrahydrofolate Dehydrogenase--biosynthesis
Transcriptional Activation--drug effects
Zymosan--chemistry