Expression, purification, and crystallization of natural and selenomethionyl recombinant ribonuclease H from Escherichia coli.

Yang, W

Expression, purification, and crystallization of natural and selenomethionyl recombinant ribonuclease H from Escherichia coli. [electronic resource] - The Journal of biological chemistry Aug 1990 - 13553-9 p. digital

Publication Type: Journal Article; Research Support, U.S. Gov't, P.H.S.

0021-9258


Chromatography, DEAE-Cellulose
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Crystallization
Enzyme Stability
Escherichia coli--genetics
Gene Expression
Mutation
Protein Conformation
Recombinant Proteins--isolation & purification
Ribonucleases--genetics
Selenium--metabolism
Selenomethionine--metabolism
Temperature
X-Ray Diffraction