Separation of post-translational modifications in monoclonal antibodies by exploiting subtle conformational changes under mildly acidic conditions.

Wang, Shiyi

Separation of post-translational modifications in monoclonal antibodies by exploiting subtle conformational changes under mildly acidic conditions. [electronic resource] - Journal of chromatography. A Oct 2010 - 6496-502 p. digital

Publication Type: Journal Article

1873-3778

10.1016/j.chroma.2010.08.044 doi


Antibodies, Monoclonal--chemistry
Calorimetry, Differential Scanning
Chromatography, High Pressure Liquid--methods
Chromatography, Ion Exchange--methods
Circular Dichroism
Glycosylation
Humans
Hydrogen-Ion Concentration
Methionine--chemistry
Models, Molecular
Oxidation-Reduction
Peptide Mapping
Protein Conformation
Protein Denaturation
Protein Processing, Post-Translational
Spectrophotometry, Ultraviolet
Temperature