Co-expression of recombinant human CYP2C9 with human cytochrome P450 reductase in protease deficient S. cerevisiae strain at a higher scale yields an enzyme of higher specific activity.

Rao, K N Purnachandra

Co-expression of recombinant human CYP2C9 with human cytochrome P450 reductase in protease deficient S. cerevisiae strain at a higher scale yields an enzyme of higher specific activity. [electronic resource] - Drug metabolism letters Dec 2010 - 246-53 p. digital

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

1874-0758

10.2174/187231210792928233 doi


Aryl Hydrocarbon Hydroxylases--antagonists & inhibitors
Cytochrome P-450 CYP2C9
Cytochromes b5--genetics
Fluorescein--metabolism
Humans
Kinetics
Microsomes--enzymology
NADPH-Ferrihemoprotein Reductase--biosynthesis
Peptide Hydrolases--deficiency
Recombinant Proteins--metabolism
Saccharomyces cerevisiae--enzymology
Substrate Specificity
Sulfaphenazole--pharmacology