Mechanistic insights into the isochorismate pyruvate lyase activity of the catalytically promiscuous PchB from combinatorial mutagenesis and selection.

Künzler, Dominik E

Mechanistic insights into the isochorismate pyruvate lyase activity of the catalytically promiscuous PchB from combinatorial mutagenesis and selection. [electronic resource] - The Journal of biological chemistry Sep 2005 - 32827-34 p. digital

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

0021-9258

10.1074/jbc.M506883200 doi


Amino Acid Sequence
Bacterial Proteins--chemistry
Binding Sites
Catalysis
Cell Proliferation
Chorismate Mutase--chemistry
Circular Dichroism
DNA-Directed RNA Polymerases--chemistry
Dose-Response Relationship, Drug
Escherichia coli--metabolism
Evolution, Molecular
Gene Expression Regulation
Gene Expression Regulation, Enzymologic
Gene Library
Genetic Techniques
Glutamic Acid--chemistry
Glutamine--chemistry
Hydrogen-Ion Concentration
Kinetics
Models, Biological
Models, Chemical
Molecular Sequence Data
Mutagenesis
Mutagenesis, Site-Directed
Mutation
Oxo-Acid-Lyases--chemistry
Plasmids--metabolism
Protein Conformation
Protein Structure, Tertiary
Pseudomonas aeruginosa--enzymology
Sequence Homology, Amino Acid
Spectrophotometry
Viral Proteins--chemistry