Self-cloning minitransposon phoA gene-fusion system promotes the rapid genetic analysis of secreted proteins in gram-negative bacteria.

Bolton, A J

Self-cloning minitransposon phoA gene-fusion system promotes the rapid genetic analysis of secreted proteins in gram-negative bacteria. [electronic resource] - BioTechniques Sep 2000 - 470-2, 474 p. digital

Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.

0736-6205

10.2144/00293bm12 doi


Alkaline Phosphatase--genetics
Bacterial Proteins--genetics
Cloning, Molecular
Cyclin-Dependent Kinases--genetics
DNA Restriction Enzymes--metabolism
Deoxyribonucleases, Type II Site-Specific
Escherichia coli--genetics
Escherichia coli Proteins
Gram-Negative Bacteria--genetics
Mutagenesis
Polymerase Chain Reaction
Recombinant Fusion Proteins