Use of modified BL21(DE3) Escherichia coli cells for high-level expression of recombinant peanut allergens affected by poor codon usage.

Kleber-Janke, T

Use of modified BL21(DE3) Escherichia coli cells for high-level expression of recombinant peanut allergens affected by poor codon usage. [electronic resource] - Protein expression and purification Aug 2000 - 419-24 p. digital

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

1046-5928

10.1006/prep.2000.1265 doi


2S Albumins, Plant
Allergens--biosynthesis
Antigens, Plant
Arachis
Blotting, Western
Cell Fractionation
Chromatography, Affinity
Cloning, Molecular
Codon
Electrophoresis, Polyacrylamide Gel
Escherichia coli--genetics
Food Hypersensitivity
Glycoproteins--biosynthesis
Humans
Immunoglobulin E--blood
Membrane Proteins
Plant Proteins--biosynthesis
Recombinant Fusion Proteins--biosynthesis