Cloning and characterization of a Saccharomyces cerevisiae alkaline ceramidase with specificity for dihydroceramide.

Mao, C

Cloning and characterization of a Saccharomyces cerevisiae alkaline ceramidase with specificity for dihydroceramide. [electronic resource] - The Journal of biological chemistry Oct 2000 - 31369-78 p. digital

Publication Type: Journal Article; Research Support, U.S. Gov't, P.H.S.

0021-9258

10.1074/jbc.M003683200 doi


Amidohydrolases--chemistry
Amino Acid Sequence
Animals
Blotting, Western
Ceramidases
Ceramides--metabolism
Codon
DNA Restriction Enzymes--metabolism
Databases, Factual
Electrophoresis, Polyacrylamide Gel
Endoplasmic Reticulum--metabolism
Escherichia coli--metabolism
Fatty Acids--metabolism
Gene Deletion
Green Fluorescent Proteins
Hot Temperature
Hydrogen-Ion Concentration
Hydrolysis
Luminescent Proteins--metabolism
Molecular Sequence Data
Mutagenesis, Site-Directed
Palmitic Acid--metabolism
Plasmids--metabolism
Saccharomyces cerevisiae--enzymology
Saccharomyces cerevisiae Proteins--chemistry
Sequence Homology, Amino Acid
Sphingosine--analogs & derivatives
Substrate Specificity
Temperature