The mechanism of the amidases: mutating the glutamate adjacent to the catalytic triad inactivates the enzyme due to substrate mispositioning. [electronic resource]
Producer: 20131203Description: 28514-23 p. digitalISSN:- 1083-351X
- Acrylamide -- metabolism
- Amidohydrolases -- chemistry
- Biocatalysis
- Catalytic Domain
- Crystallography, X-Ray
- Cysteine -- metabolism
- Enzyme Activation
- Enzyme Stability
- Geobacillus -- enzymology
- Glutamic Acid -- genetics
- Mass Spectrometry
- Mutant Proteins -- chemistry
- Mutation -- genetics
- Oxidation-Reduction
- Substrate Specificity
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Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
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