Correction of a mineralization defect by overexpression of a wild-type cDNA for COL1A1 in marrow stromal cells (MSCs) from a patient with osteogenesis imperfecta: a strategy for rescuing mutations that produce dominant-negative protein defects. [electronic resource]
Producer: 20051114Description: 1119-25 p. digitalISSN:- 0969-7128
- Bone Marrow Cells -- metabolism
- Calcification, Physiologic
- Cell Differentiation
- Cells, Cultured
- Child
- Collagen Type I -- biosynthesis
- Collagen Type I, alpha 1 Chain
- DNA, Complementary -- genetics
- Genetic Therapy -- methods
- Humans
- Male
- Mutation
- Osteoblasts -- pathology
- Osteogenesis Imperfecta -- metabolism
- Polymerase Chain Reaction -- methods
- Procollagen -- biosynthesis
- RNA, Messenger -- genetics
- Stromal Cells -- metabolism
- Transfection
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Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
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